The Jungfraujoch is a col or saddle between the Mönch and the Jungfrau in the Bernese Alps on the boundary between the cantons of Bern and Valais, inside the Jungfrau-Aletsch Protected Area.

Strictly, the Jungfraujoch is the lowest point on the mountain ridge between Mönch and Jungfrau, at 3,466 meters (11,371 ft). It is just east this location that the mountain station of Jungfraubahn is located, Jungfraujoch railway station, which at an elevation of 3,454 meters (11,332 ft) is the highest railway station in Europe. The Jungfraujoch railway station is connected to the Top of Europe building.

The Sphinx (3,571 meters or 11,716 feet) is a peak that lies just to the east of the col. It begins from the Jungfraujoch on the Valais side and at the Great Aletsch Glacier. There is an elevator to its summit, where a small viewing platform and a scientific observatory, the Sphinx Observatory, are located. Europe's highest radio relay station, the Richtfunkstation Jungfraujoch, is installed atop the ridge to the west of the railway station.

The Jungfraujoch houses one of the Global Atmosphere Watch's atmospheric research stations. The Jungfraujoch can only be reached by non-mountaineers through a 7.3-kilometre-long (4.5 mi) cog railway tunnel, served by the Jungfraubahn, the highest in a series of cooperating railway companies that provide access to the Jungfraujoch from Interlaken.